Composite

Part:BBa_K3772012:Design

Designed by: Xiaoyu Wu   Group: iGEM21_SCUT-China   (2021-10-01)


SED1Lp-VS-SAG1t expression cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 343
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 343
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 343
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 343
    Illegal AgeI site found at 1868
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is codon optimized by Sangon Biotech. (https://www.sangon.com/services_gene_mmzyhhjysjfw.html) The parts are connected together by enzyme digestion



Source

SED1Lp and SAG1t are cloned from the promoter of the coding sequence for aldehyde dehydrogenase from the genome of saccharomyces cereviciae BJ5464-NpgA； VS is synthesized by Sangon Biotech according to GenBank:JX040471.1；


References